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ptdser mrfp lact c2 biosensors  (Addgene inc)


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    Structured Review

    Addgene inc ptdser mrfp lact c2 biosensors
    Ptdser Mrfp Lact C2 Biosensors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/er+mrfp/ER-mRFP+(Plasmid+%2362236)/pmc11982563-562-6-11
    Average 93 stars, based on 12 article reviews
    ptdser mrfp lact c2 biosensors - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Sequencing:

    Article Title: Targeting Cholesterol Biosynthesis with Statins Synergizes with AKT Inhibitors in Triple-Negative Breast Cancer
    Article Snippet: The following 444 primers were generated using Agilent’s QuikChange Primer Design tool: H866A: 5’445 CAGGACATCTTGTCAAAAGTGCCATGATTCACAACAGGTCGA-3’ (forward), 5’446 TCGACCTGTTGTGAATCATGGCACTTTTGACAAGATGTCCTG-3’ (reverse), E559A: 5’447 CCAATGGCAACAACAGCAGGTTGTCTTGTGGCC-3’ (forward), 5’448 GGCCACAAGACAACCTGCTGTTGTTGCCATTGG-3’ (reverse), K691A: 5’449 GTTAGTGGTAACTATTGTACTGACGCGAAACCTGCTGCTATAAATTGGAT-3’ (forward), 5’450 ATCCAATTTATAGCAGCAGGTTTCGCGTCAGTACAATAGTTACCACTAAC-3’ (reverse), D767A: 5’451 CATTGCCTGTGGACAGGCTGCAGCACAGAATGTTG-3’ (forward), 5’452 CAACATTCTGTGCTGCAGCCTGTCCACAGGCAATG-3’ (reverse). .. To generate pLenti6-ER-mRFP, 453 mRFP with a C-terminal KDEL sequence (ER-mRFP) was PCR amplified out of ER-mRFP (Addgene, 454 62236) using the forward primer 5’-GGTCGGATCCGCCGCCACCATGGACAGCAAAGG-3’ and the 455 reverse primer 5’-GAGGCACCGGTGTTTAGAGCTCATCTTT-3’. .. PCR-amplified ER-mRFP was cloned 456 into pLenti6/V5-p53_wt p53 (gift from the Muranen lab) by double restriction enzyme digestion with 457 BamHI-HF (New England Biolabs, R0136) and AgeI-HF (New England Biolabs, R3552) at 37°C for 1 458 hour, followed by ligation with T4 DNA ligase (New England Biolabs, M0202) following the manufacturer’s 459 protocol.

    Article Title: Targeting Cholesterol Biosynthesis with Statins Synergizes with AKT Inhibitors in Triple-Negative Breast Cancer
    Article Snippet: The following primers were generated using Agilent’s QuikChange Primer Design tool: H866A: 5′-CAG GAC ATC TTG TCA AAA GTG CCA TGA TTC ACA ACA GGT CGA-3′ (forward), 5′-TCG ACC TGT TGT GAA TCA TGG CAC TTT TGA CAA GAT GTC CTG-3′ (reverse), E559A: 5′-CCA ATG GCA ACA ACA GCA GGT TGT CTT GTG GCC-3′ (forward), 5′-GGC CAC AAG ACA ACC TGC TGT TGT TGC CAT TGG-3′ (reverse), K691A: 5′-GTT AGT GGT AAC TAT TGT ACT GAC GCG AAA CCT GCT GCT ATA AAT TGG AT-3′ (forward), 5′-ATC CAA TTT ATA GCA GCA GGT TTC GCG TCA GTA CAA TAG TTA CCA CTA AC-3′ (reverse), D767A: 5′-CAT TGC CTG TGG ACA GGC TGC AGC ACA GAA TGT TG-3′ (forward), 5′-CAA CAT TCT GTG CTG CAG CCT GTC CAC AGG CAA TG-3′ (reverse). .. To generate pLenti6-ER-mRFP, mRFP with a C-terminal KDEL sequence (ER-mRFP) was PCR amplified out of ER-mRFP (Addgene, 62236) using the forward primer 5′-GGT CGG ATC CGC CGC CAC CAT GGA CAG CAA AGG-3′ and the reverse primer 5′-GAG GCA CCG GTG TTT AGA GCT CAT CTT T-3′. .. PCR-amplified ER-mRFP was cloned into pLenti6/V5-p53_wt p53 (gift from the Muranen lab) by double restriction enzyme digestion with BamHI-HF (New England Biolabs, R0136) and AgeI-HF (New England Biolabs, R3552) at 37°C for 1 hour, followed by ligation with T4 DNA ligase (New England Biolabs, M0202) by following the manufacturer’s protocol.

    Article Title: Cholesterol biosynthesis inhibition synergizes with AKT inhibitors in triple-negative breast cancer
    Article Snippet: The following primers were generated using Agilent’s QuikChange Primer Design tool: H866A: 5’- CAGGACATCTTGTCAAAAGTGCCATGATTCACAACAGGTCGA-3’ (forward), 5’- TCGACCTGTTGTGAATCATGGCACTTTTGACAAGATGTCCTG-3’ (reverse), E559A: 5’- CCAATGGCAACAACAGCAGGTTGTCTTGTGGCC-3’ (forward), 5’- GGCCACAAGACAACCTGCTGTTGTTGCCATTGG-3’ (reverse), K691A: 5’- GTTAGTGGTAACTATTGTACTGACGCGAAACCTGCTGCTATAAATTGGAT-3’ (forward), 5’- ATCCAATTTATAGCAGCAGGTTTCGCGTCAGTACAATAGTTACCACTAAC-3’ (reverse), D767A: 5’- CATTGCCTGTGGACAGGCTGCAGCACAGAATGTTG-3’ (forward), 5’- CAACATTCTGTGCTGCAGCCTGTCCACAGGCAATG-3’ (reverse). .. To generate pLenti6-ER-mRFP, mRFP with a C-terminal KDEL sequence (ER-mRFP) was PCR amplified out of ER-mRFP (Addgene, 62236) using the forward primer 5’-GGTCGGATCCGCCGCCACCATGGACAGCAAAGG-3’ and the reverse primer 5’-GAGGCACCGGTGTTTAGAGCTCATCTTT-3’. .. PCR-amplified ER-mRFP was cloned into pLenti6/V5-p53_wt p53 (gift from the Muranen lab) by double restriction enzyme digestion with BamHI-HF (New England Biolabs, R0136) and AgeI-HF (New England Biolabs, R3552) at 37°C for 1 hour, followed by ligation with T4 DNA ligase (New England Biolabs, M0202) following the manufacturer’s protocol.

    Polymerase Chain Reaction:

    Article Title: Targeting Cholesterol Biosynthesis with Statins Synergizes with AKT Inhibitors in Triple-Negative Breast Cancer
    Article Snippet: The following 444 primers were generated using Agilent’s QuikChange Primer Design tool: H866A: 5’445 CAGGACATCTTGTCAAAAGTGCCATGATTCACAACAGGTCGA-3’ (forward), 5’446 TCGACCTGTTGTGAATCATGGCACTTTTGACAAGATGTCCTG-3’ (reverse), E559A: 5’447 CCAATGGCAACAACAGCAGGTTGTCTTGTGGCC-3’ (forward), 5’448 GGCCACAAGACAACCTGCTGTTGTTGCCATTGG-3’ (reverse), K691A: 5’449 GTTAGTGGTAACTATTGTACTGACGCGAAACCTGCTGCTATAAATTGGAT-3’ (forward), 5’450 ATCCAATTTATAGCAGCAGGTTTCGCGTCAGTACAATAGTTACCACTAAC-3’ (reverse), D767A: 5’451 CATTGCCTGTGGACAGGCTGCAGCACAGAATGTTG-3’ (forward), 5’452 CAACATTCTGTGCTGCAGCCTGTCCACAGGCAATG-3’ (reverse). .. To generate pLenti6-ER-mRFP, 453 mRFP with a C-terminal KDEL sequence (ER-mRFP) was PCR amplified out of ER-mRFP (Addgene, 454 62236) using the forward primer 5’-GGTCGGATCCGCCGCCACCATGGACAGCAAAGG-3’ and the 455 reverse primer 5’-GAGGCACCGGTGTTTAGAGCTCATCTTT-3’. .. PCR-amplified ER-mRFP was cloned 456 into pLenti6/V5-p53_wt p53 (gift from the Muranen lab) by double restriction enzyme digestion with 457 BamHI-HF (New England Biolabs, R0136) and AgeI-HF (New England Biolabs, R3552) at 37°C for 1 458 hour, followed by ligation with T4 DNA ligase (New England Biolabs, M0202) following the manufacturer’s 459 protocol.

    Article Title: Targeting Cholesterol Biosynthesis with Statins Synergizes with AKT Inhibitors in Triple-Negative Breast Cancer
    Article Snippet: The following primers were generated using Agilent’s QuikChange Primer Design tool: H866A: 5′-CAG GAC ATC TTG TCA AAA GTG CCA TGA TTC ACA ACA GGT CGA-3′ (forward), 5′-TCG ACC TGT TGT GAA TCA TGG CAC TTT TGA CAA GAT GTC CTG-3′ (reverse), E559A: 5′-CCA ATG GCA ACA ACA GCA GGT TGT CTT GTG GCC-3′ (forward), 5′-GGC CAC AAG ACA ACC TGC TGT TGT TGC CAT TGG-3′ (reverse), K691A: 5′-GTT AGT GGT AAC TAT TGT ACT GAC GCG AAA CCT GCT GCT ATA AAT TGG AT-3′ (forward), 5′-ATC CAA TTT ATA GCA GCA GGT TTC GCG TCA GTA CAA TAG TTA CCA CTA AC-3′ (reverse), D767A: 5′-CAT TGC CTG TGG ACA GGC TGC AGC ACA GAA TGT TG-3′ (forward), 5′-CAA CAT TCT GTG CTG CAG CCT GTC CAC AGG CAA TG-3′ (reverse). .. To generate pLenti6-ER-mRFP, mRFP with a C-terminal KDEL sequence (ER-mRFP) was PCR amplified out of ER-mRFP (Addgene, 62236) using the forward primer 5′-GGT CGG ATC CGC CGC CAC CAT GGA CAG CAA AGG-3′ and the reverse primer 5′-GAG GCA CCG GTG TTT AGA GCT CAT CTT T-3′. .. PCR-amplified ER-mRFP was cloned into pLenti6/V5-p53_wt p53 (gift from the Muranen lab) by double restriction enzyme digestion with BamHI-HF (New England Biolabs, R0136) and AgeI-HF (New England Biolabs, R3552) at 37°C for 1 hour, followed by ligation with T4 DNA ligase (New England Biolabs, M0202) by following the manufacturer’s protocol.

    Article Title: Cholesterol biosynthesis inhibition synergizes with AKT inhibitors in triple-negative breast cancer
    Article Snippet: The following primers were generated using Agilent’s QuikChange Primer Design tool: H866A: 5’- CAGGACATCTTGTCAAAAGTGCCATGATTCACAACAGGTCGA-3’ (forward), 5’- TCGACCTGTTGTGAATCATGGCACTTTTGACAAGATGTCCTG-3’ (reverse), E559A: 5’- CCAATGGCAACAACAGCAGGTTGTCTTGTGGCC-3’ (forward), 5’- GGCCACAAGACAACCTGCTGTTGTTGCCATTGG-3’ (reverse), K691A: 5’- GTTAGTGGTAACTATTGTACTGACGCGAAACCTGCTGCTATAAATTGGAT-3’ (forward), 5’- ATCCAATTTATAGCAGCAGGTTTCGCGTCAGTACAATAGTTACCACTAAC-3’ (reverse), D767A: 5’- CATTGCCTGTGGACAGGCTGCAGCACAGAATGTTG-3’ (forward), 5’- CAACATTCTGTGCTGCAGCCTGTCCACAGGCAATG-3’ (reverse). .. To generate pLenti6-ER-mRFP, mRFP with a C-terminal KDEL sequence (ER-mRFP) was PCR amplified out of ER-mRFP (Addgene, 62236) using the forward primer 5’-GGTCGGATCCGCCGCCACCATGGACAGCAAAGG-3’ and the reverse primer 5’-GAGGCACCGGTGTTTAGAGCTCATCTTT-3’. .. PCR-amplified ER-mRFP was cloned into pLenti6/V5-p53_wt p53 (gift from the Muranen lab) by double restriction enzyme digestion with BamHI-HF (New England Biolabs, R0136) and AgeI-HF (New England Biolabs, R3552) at 37°C for 1 hour, followed by ligation with T4 DNA ligase (New England Biolabs, M0202) following the manufacturer’s protocol.

    Amplification:

    Article Title: Targeting Cholesterol Biosynthesis with Statins Synergizes with AKT Inhibitors in Triple-Negative Breast Cancer
    Article Snippet: The following 444 primers were generated using Agilent’s QuikChange Primer Design tool: H866A: 5’445 CAGGACATCTTGTCAAAAGTGCCATGATTCACAACAGGTCGA-3’ (forward), 5’446 TCGACCTGTTGTGAATCATGGCACTTTTGACAAGATGTCCTG-3’ (reverse), E559A: 5’447 CCAATGGCAACAACAGCAGGTTGTCTTGTGGCC-3’ (forward), 5’448 GGCCACAAGACAACCTGCTGTTGTTGCCATTGG-3’ (reverse), K691A: 5’449 GTTAGTGGTAACTATTGTACTGACGCGAAACCTGCTGCTATAAATTGGAT-3’ (forward), 5’450 ATCCAATTTATAGCAGCAGGTTTCGCGTCAGTACAATAGTTACCACTAAC-3’ (reverse), D767A: 5’451 CATTGCCTGTGGACAGGCTGCAGCACAGAATGTTG-3’ (forward), 5’452 CAACATTCTGTGCTGCAGCCTGTCCACAGGCAATG-3’ (reverse). .. To generate pLenti6-ER-mRFP, 453 mRFP with a C-terminal KDEL sequence (ER-mRFP) was PCR amplified out of ER-mRFP (Addgene, 454 62236) using the forward primer 5’-GGTCGGATCCGCCGCCACCATGGACAGCAAAGG-3’ and the 455 reverse primer 5’-GAGGCACCGGTGTTTAGAGCTCATCTTT-3’. .. PCR-amplified ER-mRFP was cloned 456 into pLenti6/V5-p53_wt p53 (gift from the Muranen lab) by double restriction enzyme digestion with 457 BamHI-HF (New England Biolabs, R0136) and AgeI-HF (New England Biolabs, R3552) at 37°C for 1 458 hour, followed by ligation with T4 DNA ligase (New England Biolabs, M0202) following the manufacturer’s 459 protocol.

    Article Title: Targeting Cholesterol Biosynthesis with Statins Synergizes with AKT Inhibitors in Triple-Negative Breast Cancer
    Article Snippet: The following primers were generated using Agilent’s QuikChange Primer Design tool: H866A: 5′-CAG GAC ATC TTG TCA AAA GTG CCA TGA TTC ACA ACA GGT CGA-3′ (forward), 5′-TCG ACC TGT TGT GAA TCA TGG CAC TTT TGA CAA GAT GTC CTG-3′ (reverse), E559A: 5′-CCA ATG GCA ACA ACA GCA GGT TGT CTT GTG GCC-3′ (forward), 5′-GGC CAC AAG ACA ACC TGC TGT TGT TGC CAT TGG-3′ (reverse), K691A: 5′-GTT AGT GGT AAC TAT TGT ACT GAC GCG AAA CCT GCT GCT ATA AAT TGG AT-3′ (forward), 5′-ATC CAA TTT ATA GCA GCA GGT TTC GCG TCA GTA CAA TAG TTA CCA CTA AC-3′ (reverse), D767A: 5′-CAT TGC CTG TGG ACA GGC TGC AGC ACA GAA TGT TG-3′ (forward), 5′-CAA CAT TCT GTG CTG CAG CCT GTC CAC AGG CAA TG-3′ (reverse). .. To generate pLenti6-ER-mRFP, mRFP with a C-terminal KDEL sequence (ER-mRFP) was PCR amplified out of ER-mRFP (Addgene, 62236) using the forward primer 5′-GGT CGG ATC CGC CGC CAC CAT GGA CAG CAA AGG-3′ and the reverse primer 5′-GAG GCA CCG GTG TTT AGA GCT CAT CTT T-3′. .. PCR-amplified ER-mRFP was cloned into pLenti6/V5-p53_wt p53 (gift from the Muranen lab) by double restriction enzyme digestion with BamHI-HF (New England Biolabs, R0136) and AgeI-HF (New England Biolabs, R3552) at 37°C for 1 hour, followed by ligation with T4 DNA ligase (New England Biolabs, M0202) by following the manufacturer’s protocol.

    Article Title: Cholesterol biosynthesis inhibition synergizes with AKT inhibitors in triple-negative breast cancer
    Article Snippet: The following primers were generated using Agilent’s QuikChange Primer Design tool: H866A: 5’- CAGGACATCTTGTCAAAAGTGCCATGATTCACAACAGGTCGA-3’ (forward), 5’- TCGACCTGTTGTGAATCATGGCACTTTTGACAAGATGTCCTG-3’ (reverse), E559A: 5’- CCAATGGCAACAACAGCAGGTTGTCTTGTGGCC-3’ (forward), 5’- GGCCACAAGACAACCTGCTGTTGTTGCCATTGG-3’ (reverse), K691A: 5’- GTTAGTGGTAACTATTGTACTGACGCGAAACCTGCTGCTATAAATTGGAT-3’ (forward), 5’- ATCCAATTTATAGCAGCAGGTTTCGCGTCAGTACAATAGTTACCACTAAC-3’ (reverse), D767A: 5’- CATTGCCTGTGGACAGGCTGCAGCACAGAATGTTG-3’ (forward), 5’- CAACATTCTGTGCTGCAGCCTGTCCACAGGCAATG-3’ (reverse). .. To generate pLenti6-ER-mRFP, mRFP with a C-terminal KDEL sequence (ER-mRFP) was PCR amplified out of ER-mRFP (Addgene, 62236) using the forward primer 5’-GGTCGGATCCGCCGCCACCATGGACAGCAAAGG-3’ and the reverse primer 5’-GAGGCACCGGTGTTTAGAGCTCATCTTT-3’. .. PCR-amplified ER-mRFP was cloned into pLenti6/V5-p53_wt p53 (gift from the Muranen lab) by double restriction enzyme digestion with BamHI-HF (New England Biolabs, R0136) and AgeI-HF (New England Biolabs, R3552) at 37°C for 1 hour, followed by ligation with T4 DNA ligase (New England Biolabs, M0202) following the manufacturer’s protocol.

    Generated:

    Article Title: Motion of single molecular tethers reveals dynamic subdomains at ER-mitochondria contact sites
    Article Snippet: .. ER-mRFP (Addgene #62236), mTagRFP-T2-Mito-7 (Addgene #58041) (referred to as mitoRFP in the text), mTagBFP2-N1 (Addgene #54566), mEGFP-N1 (Addgene #54767), mEGFP-C1 (Addgene #54759), and mEmerald-Sec61b-C1 (Addgene #90992) have been described previously, and were gifts from Erik Snapp, Michael Davidson, or generated in house. ..

    Article Title: Motion of VAPB molecules reveals ER–mitochondria contact site subdomains
    Article Snippet: .. ER-mRFP (Addgene no. 62236), mTagRFP-T2-Mito-7 (Addgene no. 58041) (referred to as mitoRFP in the text), mTagBFP2-N1 (Addgene no. 54566), mEGFP-N1 (Addgene no. 54767), mEGFP-C1 (Addgene no. 54759) and mEmerald-Sec61b-C1 (Addgene no. 90992) have been described previously, and were gifts from Erik Snapp, Michael Davidson, or generated in house. ..

    Article Title: Motion of VAPB molecules reveals ER-mitochondria contact site subdomains.
    Article Snippet: .. ER-mRFP (Addgene no. 62236), mTagRFP-T2-Mito-7 (Addgene no. 58041) (referred to as mitoRFP in the text), mTagBFP2-N1 (Addgene no. 54566), mEGFP-N1 (Addgene no. 54767), mEGFP-C1 (Addgene no. 54759) and mEmerald-Sec61b-C1 (Addgene no. 90992) have been described previously, and were gifts from Erik Snapp, Michael Davidson, or generated in house. ..



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